Rhizoma coptidis Inhibits LPS-Induced MCP-1/CCL2 Production in Murine Macrophages via an AP-1 and NF𝜅B-Dependent Pathway

Figure 4

The main alkaloid compound of Rhizoma coptidis, berberine, inhibited binding activity of AP-1 (a) and NF$\kappa$ B (b) in a concentration dependent fashion. Inhibitory potential of berberine was directly compared with the total extract of Rhizoma coptidis (dilution $1:5$, incubation time 240 min). LPS-stimulated cells were incubated for 240 min. Berberine concentrations listed represent mol. AP-1: activated protein-1, NF$\kappa$ B: nuclear factorκB, Co: LPS-stimulated control cells, RC:Rhizoma coptidis, Be: berberine, and CC: cold competition. ${}^{*}P<.05\text{,}$${}^{**}P<.001$.

(a)

(b)