Rhizoma coptidis Inhibits LPS-Induced MCP-1/CCL2 Production in Murine Macrophages via an AP-1 and NF𝜅B-Dependent Pathway

Figure 6

Secrection of MCP-1/CCL2 was evaluated with ELISAs of supernatants. Supernatants were collected after preincubation with different concentrations (a) and exposure times (b) of Rhizoma coptidis. Berberine also inhibited MCP-1/CCL2 secretion in a concentration-dependent fashion (c). Berberine concentrations listed represent mol. LPS: lipopolysaccharide, RC:Rhizoma coptidis, Be: berberine, MCP-1/CCL2: monocyte chemoattractant protein-1. ${}^{*}P<.005\text{,}$${}^{**}P<.001$. AP-1: activated protein-1, Co: LPS-stimulated control cells, and CC: cold competition. ${}^{†}P<.05\text{,}$${}^{*}P<.005\text{,}$${}^{**}P<.001$.

(a)

(b)

(c)