Mediators of Inflammation

Research Article

Role of Meconium and Hypoxia in Meconium Aspiration-Induced Lung Injury in Neonatal Rabbits

Table 3

(a) Expression of TNFα, IL-1β, IL-6, and IL-10 cytokines after saline, meconium, or milk instillation (in pg/ml).
Sal 8 hrsMec 4 hrsMec 8 hrsMec 24 hrsMilk 4 hrsMilk 8 hrsMilk 24 hrs
TNFα ( 𝑃 < . 0 5 ) 0 . 3 9 ± 0 . 1 1 0 . 8 0 ± 0 . 3 2 1 . 4 5 ± 0 . 8 4 1 . 2 4 ± 0 . 4 2 0 . 4 0 ± 0 . 1 1 0 . 4 3 ± 0 . 2 1 0 . 6 4 ± 0 . 1 8
IL-1β 0 . 2 9 ± 0 . 1 9 0 . 4 4 ± 0 . 2 2 0 . 3 3 ± 0 . 1 5 0 . 2 5 ± 0 . 1 2 0 . 1 2 ± 0 . 0 5 0 . 1 7 ± 0 . 0 5 0 . 6 5 ± 0 . 1 2
IL-6 ( 𝑃 < . 0 5 ) 0 . 2 1 ± 0 . 1 4 0 . 4 4 ± 0 . 2 5 0 . 9 2 ± 0 . 3 4 0 . 8 1 ± 0 . 2 6 0 . 2 4 ± 0 . 0 5 0 . 1 9 ± 0 . 1 0 0 . 6 8 ± 0 . 1 2
IL-10 0 . 2 5 ± 0 . 0 9 0 . 2 3 ± 0 . 1 2 0 . 2 1 ± 0 . 1 7 0 . 2 7 ± 0 . 1 9 0 . 3 1 ± 0 . 2 7 0 . 1 6 ± 0 . 1 7 0 . 2 8 ± 0 . 1 4
Changes in cytokine levels within the saline-treated or milk-treated groups were not significant. In meconium group, cytokine levels increased significantly at 8 and 24 hrs.
(b) Expression of TNFα, IL-1β, IL-6, and IL-10 cytokines after 15-minute treatment in hypoxic or hyperoxic conditions.
Cytokines (pg/ml)Saline 8 hrsHypoxia 15 minHyperoxia 15 min
TNFα ( 𝑃 < . 0 5 ) 0 . 3 9 ± 0 . 1 1 0 . 5 4 ± 0 . 2 9 0 . 7 1 ± 0 . 2 1
IL-1β 0 . 2 9 ± 0 . 1 9 0 . 4 4 ± 0 . 1 5 0 . 4 0 ± 0 . 1 7
IL-6 ( 𝑃 < . 0 5 ) 0 . 2 1 ± 0 . 1 4 0 . 6 9 ± 0 . 2 0 0 . 6 7 ± 0 . 3 6
IL-10 0 . 3 5 ± 0 . 1 9 0 . 4 3 ± 0 . 3 9 0 . 3 9 ± 0 . 2 0
Cytokine levels in hypoxic or hyperoxic conditions were elevated. However, a significant increase was observed only for TNFα and IL6 expressions for both hypoxic and hyperoxic groups compared to saline-instilled group.