Circulating Endothelial Microparticles in Diabetes Mellitus
Figure 2
Gating protocol. (a) Bivariant analysis efficiently distinguished EMPs from quantitation beads. (b) EMPs from activated EC culture had negligible autofluorescence on FITC/PE channels. (c) Two-color staining with CD41a enables CD31+ EMPs to be segregated from platelet microparticles. (d) Logicle displays provide improved representation of the EMP population with minimal fluorescence.
