Mediators of Inflammation

Research Article

High-Fat Diet-Induced Neuropathy of Prediabetes and Obesity: Effect of PMI-5011, an Ethanolic Extract of Artemisia dracunculus L.

Variables of small sensory fiber neuropathy in normal chow and high fat diet-fed mice maintained with and without PMI-5011 treatment.


Groups	NC	$NC + T$	HFD	$HFD + T$

Variables
Baseline (prior to the beginning of HFD feeding)

Paw withdrawal latency, s⁻¹	$8.8 \pm 0.15$
Tail flick response latency, s⁻¹	$2.41 \pm 0.08$
Tactile withdrawal threshold, g	$1.76 \pm 0.12$
Mechanical withdrawal threshold, g	$11.5 \pm 0.31$

16-wk time point (prior to PMI-5011 treatment)

Paw withdrawal latency, s⁻¹	$8.2 \pm 0.17$		$13.0 \pm 0.31^{* *}$
Tail flick response latency, s⁻¹	$2.48 \pm 0.09$		$4.62 \pm 0.13^{* *}$
Tactile withdrawal threshold, g	$1.72 \pm 0.13$		$1.06 \pm 0.09^{* *}$
Mechanical withdrawal threshold, g	$10.6 \pm 0.28$		$13.3 \pm 0.24^{* *}$

23-wk time point (final measurements)

Paw withdrawal latency, s⁻¹	$9.1 \pm 0.32$	$10.3 \pm 0.41$	$15.7 \pm 1.14^{* *}$	$11.4 \pm 0.68^{§, *, # #}$
Tail flick response latency, s⁻¹	$2.29 \pm 0.11$	$2.52 \pm 0.10$	$4.07 \pm 0.15^{* *}$	$2.59 \pm 0.10^{§ §, # #}$
Tactile withdrawal threshold, g	$1.52 \pm 0.21$	$1.45 \pm 0.16$	$0.76 \pm 0.03^{* *}$	$1.20 \pm 0.08^{£ £, #}$
Mechanical withdrawal threshold, g	$11.2 \pm 0.46$	$11.2 \pm 0.36$	$15.2 \pm 0.10^{* *}$	$12.3 \pm 0.59^{£, # #}$

Data are expressed as

Mean \pm SEM,

n = 10 – 12

per group. NC: normal chow; T: treatment; HFD: high fat diet;

^{*, * *} P < . 05

and

< . 01

versus corresponding controls;

^{§, § §} P < . 05

and

< . 01

versus the baseline value (16-wk time point);

^{£, £ £} P < . 05

and

< . 01

versus mice fed NC (16-wk time point);

^{#, # #} P < . 05

and

< . 01

versus untreated mice fed HFD (23-wk time point).