Effects of Brain-Derived Neurotrophic Factor on Local Inflammation in Experimental Stroke of Rat
Figure 6
Effect of BDNF on mRNA expression of cytokine brain homogenates were obtained from ischemic cortexes 6 h and 24 h after reperfusion. The expressions of mRNA of interleukin10 (IL10) and tumor necrosis factor α (TNF-α) in rat brain after stroke were measured by real-time quantitative polymerase chain reaction (PCR). (a) Levels of IL10 mRNA. Application of BDNF antibody prevented upregulation of IL10 mRNA 6 h after reperfusion. At 24 h of reperfusion, BDNF increased level of mRNA of IL10 by 1.93 fold. (b) Levels if TNF-α mRNA. Exogenous BDNF significantly decreased mRNA of TNF-α 6 h and 24 h after reperfusion. Inhibition of endogenous BDNF significantly increased the expression of TNF-α 6 h after reperfusion. Bars represent mean ± SD (). versus control group, versus BDNF group.
