Interleukin-6 Contributes to Age-Related Alteration of Cytokine Production by Macrophages

Table 1

Effects of age and IL-6 on splenocyte cell populations.

	Cells (%)
	WT		IL-6 KO
	Young	Aged	Young	Aged
CD3^+ T Cells	$47.1\pm 1.1$	$41.1\pm {1.1}^{*}$	$48.3\pm 1.1$	$36.9\pm {0.9}^{*}$
CD3^+/CD4^+ T Cells	$34.1\pm 0.9$	$31.0\pm 0.8$	$32.9\pm 0.8$	$30.1\pm 1.7$
CD3^+/CD8^+T Cells	$13.6\pm 0.9$	$10.2\pm {0.7}^{*}$	$16.6\pm {0.4}^{**}$	$8.8\pm {0.2}^{*}$
Ly49c^+NK Cells	$5.4\pm 0.1$	$4.2\pm {0.3}^{*}$	$5.4\pm 0.2$	$4.1\pm {0.2}^{*}$
F4/80^+Macrophages	$15.9\pm 2.1$	$12.6\pm 1.2$	$14.1\pm 2.3$	$13.3\pm 0.9$
CD19^+B cells	$41.1\pm 1.5$	$43.4\pm 1.2$	$43.6\pm 2.3$	$46.9\pm 0.9$

Splenocytes were incubated with anti-CD3, CD4, CD8, Ly49c, F4/80, or CD19 antibodies and analyzed by flow cytometry. Data are shown as mean ± SEM of 4-5 mice per group. ${}^{*}P<.05$ from aged matched control. ${}^{**}P<.05$ from young WT.