Role of Bone Marrow-Derived Monocytes/Macrophages in the Repair of Mucosal Damage Caused by Irradiation and/or Anticancer Drugs in Colitis Model

Figure 3

Flow cytometric analysis of peripheral blood monocyte subsets. Five mice were sacrificed at each time point after RT, and whole peripheral blood was pooled. Peripheral blood mononuclear cells (PBMC) were isolated from whole blood using gradient centrifugation method. 1×10⁶ PBMC were stained using two antibodies, CD11b-PerCP-Cy5.5 and CD62L-APC. CD11b⁺CD62L⁺ population was designated as Subset 1, and CD11b⁺CD62L⁻ population was Subset 2.

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