Role of Bone Marrow-Derived Monocytes/Macrophages in the Repair of Mucosal Damage Caused by Irradiation and/or Anticancer Drugs in Colitis Model
Figure 7
F4/80+CD11b+Gr1+ (Subset 1) cells accumulated at the mucosal damaged area in early phase of healing. BMC were labeled using CFDA-SE. These cells were injected into AA + 5-FU mice via tail vein. After 24 hours, colon was dissected and incubated in calcium and magnesium-free HBSS containing 2.5% heat-inactivated FBS and 1 mM DTT to remove mucus. After the tissues were treated with collagenase and DNase I for 60 min at 37°C, cells were washed and stained with 7-AAD, F4/80-APC, and C11b-PE or 7-AAD, F4/80-APC, and Gr1-PE, for the flow cytometric analysis. 7-AAD was used for exclusion of dead cells. (a) CFDA-SE positive cells indicated bone marrow-derived cells. (b) CFDA-SE positive population was CD11b+F4/80+ monocytes. (c) F4/80 positive population was almost all Gr1+ cells, indicating the F4/80+Gr1+ Subset 1 monocytes. (d) The population had typical monocytes morphology.
