Preferential Th1 Cytokine Profile of Phosphoantigen-Stimulated Human Vγ9Vδ2 T Cells

Figure 1

Vγ9Vδ2 T cells rapidly produce IFN-γ, but not IL-10, IL-13, nor IL-17, in response to PMA and ionomycin or pyrophosphate stimulation. (a) Flow cytometry dot plots showing IFN-γ, IL-10, IL-13, and IL-17 expression by Vγ9⁺ and Vγ9⁻ PBMC after 4-hour stimulation with PMA and ionomycin or HMB-PP, in the presence of brefeldin A. Plots are representative of experiments on PBMC from 8 donors. Numbers in the lower right hand quadrants indicate the percentages of lymphocytes that express the Vγ9 TCR. Numbers in the upper right hand quadrants indicate the percentages of Vγ9⁺ T cells that produce each cytokine.

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