Preferential Th1 Cytokine Profile of Phosphoantigen-Stimulated Human Vγ9Vδ2 T Cells

Figure 5

Cytokine expression by Vγ9Vδ2 cells is not influenced by CD4 or CD8 expression. PBMCs were stimulated with PMA and ionomycin and surface expression of Vγ9, Vδ2, CD4, and CD8, and intracellular expression of cytokines was analysed by flow cytometry after 4 hours. (a) Vγ9⁺Vδ2⁺ cells were gated initially and (b) CD4 and CD8 expression by gated Vγ9Vδ2 T cells was analysed. (c) Mean frequencies of CD4⁺, CD8⁺, and double-negative (DN) Vγ9Vδ2 T cells from 6 donors. (d, e) Flow cytometry dot plots showing expression of TNF-α by CD4⁺ and CD4⁻ (d) and CD8⁺ and CD8⁻ (e) Vδ2Vγ9 T cells stimulated with PMA and ionomycin. (f) Dot plot showing expression of TNF-α by gated DN Vγ9Vδ2 T cells. Arrows show electronic gates used to generate dot plots. (g, h) Frequencies of CD4⁺, CD8⁺, and DN Vδ2Vγ9 T cell that produced TNF-α (g) and IFN-γ (h) in response to stimulation with PMA and ionomycin. Results are means of 5 independent experiments.