Leptin Administration Downregulates the Increased Expression Levels of Genes Related to Oxidative Stress and Inflammation in the Skeletal Muscle of ob/ob Mice

Figure 2

Real-Time PCR analysis of peroxisome proliferator-activated receptor coactivator 1$\alpha$  (Pgc1a), forkhead box class O1 (Foxo1), muscle atrophy F box (MAFbx) and muscle RING finger 1 (MuRF1) in gastrocnemius muscle of wild type (open), control ob/ob (closed), pair-fed ob/ob (gray) and leptin-treated ob/ob (striped) mice ($n=5$ per group). Data are presented as $\text{mean}\pm \text{SEM}$ of the ratio between gene expression and 18S rRNA. ${}^{*}P<.05$ and ${}^{**}P<.01$ by Kruskal-Wallis followed by Mann Whitney’s $U$ test.

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