The Traditional Japanese Formula Keishibukuryogan Inhibits the Production of Inflammatory Cytokines by Dermal Endothelial Cells
Figure 1
Effect of KBG on LPS-stimulated induction of cytokines in HDMECs. (a) HDMEC cells were treated with 1 μg/mL LPS and/or KBG (10 mg/mL) for 6 or 24 h and were subjected to the MTT assay. (b) HDMEC cells were treated with 1 μg/mL LPS and/or 10 mg/mL of KBG for 24 h. The MIF, IL-6, IL-8, and TNF-α content of cultured supernatants was analyzed by ELISA. Data are presented as the means ± S.D (n = 5). *P < .001, **P < .005, ***P < .05. (c) The cells were treated with 1 μg/mL LPS and/or 10 mg/mL of KBG for 6 h. Total RNA was isolated, and the mRNA expression levels of MIF, IL-6, IL-8, and TNF-α were detected by RT-PCR. (These experiments were repeated three times with similar results).
