Mediators of Inflammation

Research Article

Normal Human Gingival Epithelial Cells Sense C. parapsilosis by Toll-Like Receptors and Module Its Pathogenesis through Antimicrobial Peptides and Proinflammatory Cytokines

Table 1

Primer sequences used for the q-RT-PCR.
Gene namePrimers sequencesProduct size (bp)
TLR-2sense: 5′-GCCTCTCCAAGGAAGAATCC-3′144
antisense: 5′-TCCTGTTGTTGGACAGGTCA-3′
TLR-4sense: 5′-AATCTAGAGCACTTGGACCTTTCC-3′116
antisense: 5′-GGGTTCAGGGACAGGTCTAAAGA-3′
TLR-6sense: 5′-CATCCTATTGTGAGTTTCAGGCAT-3′121
antisense: 5′-GCTTCATAGCACTACATCCCAAG-3′
TLR-9sense: 5′-GGACCTCTGGTACTGCTTCCA-3′151
antisense: 5′-AAGCTCGTTGTACACCCAGTCT-3′
β1-defensinsense: 5′-GCCTCTCCCCAGTTCCTGAA-3′82
antisense: 5′-GCAGAGAGTAAACAGCAGAAGGTA-3′
β2-defensinsense: 5′-TGTGGTCTCCCTGGAACAAAAT-3′105
antisense: 5′-GTCGCACGTCTCTGATGAGG-3′
β3-defensinsense: 5′-CTTCTGTTTGCTTTGCTCTTCCT-3′138
antisense: 5′-CTGTTCCTCCTTTGGAAGGCA-3′
β4-defensinsense: 5′-CACTCTACCAACACGCACCTAG-3′133
antisense: 5′-CGCAACTGGAACCACACACT-3′
IL-1βsense: 5′-CTGTCCTGCGTGTTGAAAGA-3′69
antisense: 5′-TTGGGTAATTTTTGGGATCTACA-3′
IFNγsense: 5′-GGCATTTTGAAGAATTGGAAAG-3′111
antisense: 5′-TTTGGATGCTCTGGTCATCTT-3′
TNFαsense: 5′-CAGCCTCTTCTCCTTCCTGAT-3′122
antisense: 5′-GCCAGAGGGCTGATTAGAGA-3′
GAPDHsense: 5′-GGTATCGTCGAAGGACTCATGAC-3′180
antisense: 5′-ATGCCAGTGAGCTTCCCGTTCAGC-3′