Normal Human Gingival Epithelial Cells Sense C. parapsilosis by Toll-Like Receptors and Module Its Pathogenesis through Antimicrobial Peptides and Proinflammatory Cytokines

Table 2

Growth of C. parapsilosis following contact with human oral epithelial cells, and viability of epithelial cells after contact with Candida.

Contact time (h)	Nb. of C. parapsilosis ^& ($\times$10^5/cm^2)	Viability of epithelial cells (%)
3	1.6 $\pm$ 0.7	97.3 $\pm$ 0.01
6	0.9 $\pm$ 0.03**	97.0 $\pm$ 0.02
12	0.1 $\pm$ 0.09**	96.5 $\pm$ 0.32
24	0.03 $\pm$ 0.01**	96.9 $\pm$ 0.37

${}_{}{}^{\&}C$ . parapsilosis was seeded onto epithelial cell culture at 10⁵ cells/cm². To assess the growth of Candida following contact with the epithelial cells, nonadherent (in the supernatant) and adherent (on the monolayer epithelial cell cultures) C. parapsilosis were collected and enumerated. To assess the viability of epithelial cells, cultures were treated with trypsin and cells were washed twice then viable epithelial cells were determined using trypan bleu exclusion test.
${}_{}{}^{**}P\le .01$ is level of significance for growth inhibition of C. parapsilosis in infected oral epithelial cell cultures compared to initial seeding concentration of C. parapsilosis.