Lipopolysaccharide Inhibits the Channel Activity of the P2X7 Receptor

Figure 5

LPS protects from ATP-induced depletion and apoptosis in CD4+ CD25+ T-regulatory cells. (a) LPS abolishes the ATP-induced depletion of Treg’s population. ATP (60 μM, gray bars) was applied for 30 min, 24 h before cell staining and flow cytometry. *𝑃<0.05, Mann-Whitney test, 𝑛=4. (b) Effects of LPS (1 μg/mL) and BBG (100 nM, a P2X7R antagonist) in ATP-induced early apoptosis measured with annexin V (AV) assay. ATP (60 μM) was applied for 30 min, 3 h before cell staining and flow cytometry. Open bars correspond to the apoptotic population, whereas gray bars represent necrotic cells. *𝑃<0.05, Mann-Whitney test, 𝑛=4. (c) LPS and BBG inhibit the ATP-induced increase in CD4+ CD25+, annexin-positive population. ATP was applied for 30 min, 3 h before cell staining and flow cytometry. *𝑃<0.05, Mann-Whitney test, 𝑛=4.

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