Decreased Anti-Inflammatory Responses to Vitamin D in Neonatal Neutrophils
Figure 3
1,25 vit D3 does not suppress PMA-induced H2O2 generation in adult or neonatal neutrophils. Adult (a) and neonatal (b) neutrophils (5 × 104 cells) were incubated with Amplex Red (25 M) and horseradish peroxidise and then treated with PMA (500 nM) or medium control, in the presence or absence of 1,25-vit D3 (10−7 M). H2O2 production was quantified at 1 min intervals for 30 min. Each point represents the mean ± SE (). Control, ; PMA, ◯; 1,25-Vit D3, ; PMA + 1,25-Vit D3, .