Novel Biphasic Role of LipoxinA4 on Expression of Cyclooxygenase-2 in Lipopolysaccharide-Stimulated Lung Fibroblasts
Figure 3
The effect of LXA4 on LPS-induced expression of COX-2 protein expression and PGE2 and PGD2 production at 6 hours in primary lung fibroblasts. (a) Rat lung fibroblast cells were treated with LXA4 at 0, 100, 200 or 400 nM in the presence of LPS (1 μg/mL) for 6 hours. Cells were then harvested, sonicated and COX-2 protein detected by western blot. * versus control group, #versus (100, 200 or 400 nM) LXA4 groups; & versus (200 or 400 nM) LXA4 groups. (b) Supernatants from rat lung fibroblast cells treated with LXA4 at 0, 100, 200, or 400 nM in the presence of LPS (1 μg/mL) for 6 hours were collected and PGE2 protein measured by ELISA. Data are expressed as mean ± SE for each group. * versus control group, #versus (100, 200, or 400 nM) LXA4 groups; & versus (200 or 400 nM) LXA4 groups. (c) Supernatants from rat lung fibroblast cells treated with LXA4 at 0, 100, 200 or 400 nM in the presence of LPS (1 μg/mL) for 6 hours were collected and PGD2 protein measured by ELISA. Data are expressed as mean ± SE for each group. * versus control group.
