Novel Biphasic Role of LipoxinA4 on Expression of Cyclooxygenase-2 in Lipopolysaccharide-Stimulated Lung Fibroblasts
Figure 4
The effect of LXA4 on LPS-induced expression of COX-2 protein expression and PGE2 and PGD2 production at 24 hours in primary lung fibroblasts. (a) Rat lung fibroblast cells were treated with LXA4 at 0, 100, 200, or 400 nM in the presence of LPS (1 μg/mL) for 24 hours. Cells were then harvested, sonicated, and COX-2 protein detected by Western blot. * versus control group, # versus non-LXA4 groups; & versus (100 or 200 nM) LXA4 groups. (b) Supernatants from rat lung fibroblast cells treated with LXA4 at 0, 100, 200, or 400 nM in the presence of LPS (1 μg/mL) for 24 hours were collected and PGE2 protein measured by ELISA. Data are expressed as mean SE for each group. * versus control group. (c) Supernatants from rat lung fibroblast cells treated with LXA4 at 0, 100, 200 or 400 nM in the presence of LPS (1 μg/mL) for 24 hours were collected and PGD2 protein measured by ELISA. Data are expressed as mean ± SE for each group.* versus control group, #versus (0, 100 or 200 nM) LXA4 groups.
