PAF-stimulated LC induce Th17 mRNA markers in cocultured T cells. Monocyte-derived LC were stimulated with graded concentrations of PAF or its vehicle (ethanol; V) and cocultured with antiCD3/CD28-activated CD4+ T cells for 5 days. IL-17A (a), RORC2 (b), IL-21 (c), and IL-22 (d) mRNA expression was measured by real-time quantitative PCR in CD4+ T cells. For comparison, CD4+ T cells were cultured alone with IL-1β, IL-6, and IL-23 for 5 days. Data (means ± SEM) are expressed as fold induction relative to vehicle (V) control. ; *; **.