Distinct Proteasome Subpopulations in the Alveolar Space of Patients with the Acute Respiratory Distress Syndrome
Figure 2
Representative western blots with a polyclonal antibody directed against LMP2 or LMP7 subunits of the immunoproteasome of samples of BAL supernatant obtained from healthy subjects. (a) LMP2 immunoproteasome protein could not be detected in BAL supernatant of any healthy subject. Lanes are identified as follows: Lane 1: 1 μg 20S standard proteasome (human erythrocyte); Lane 2: 1 μg immunoproteasome (human spleen); Lane 3: BAL supernatant healthy subject 1; Lane 4: BAL supernatant healthy subject 2; Lane 5: BAL supernatant healthy subject 3; Lane 6: BAL supernatant healthy subject 4; Lane 7: BAL supernatant healthy subject 5; Lane 8: BAL supernatant healthy subject 6; Lane 9: BAL supernatant healthy subject 7. (b) LMP7 immunoproteasome protein could not be detected in BAL supernatant of any healthy subject. Lanes are identified as follows. Lane 1: 1 μg 20S standard proteasome (human erythrocyte); Lane 2: 1 μg immunoproteasome (human spleen); Lane 3: BAL supernatant healthy subject 1; Lane 4: BAL supernatant healthy subject 2; Lane 5: BAL supernatant healthy subject 3; Lane 6: BAL supernatant healthy subject 4; Lane 7: BAL supernatant healthy subject 5; Lane 8: BAL supernatant healthy subject 6; Lane 9: BAL supernatant healthy subject 7.
