Figure 8: Effect of EGFR and HER2 inhibition on TNF-α-stimulated IL-8 secretion. HT-29 cells were treated with increasing doses of AG1478 for 30 mins prior to 6 hr stimulation with 10 ng/mL TNF-α (a). HT-29 cells were treated with increasing doses of the HER2 inhibitor AG879 or a combination of AG1478 and AG879 (b). Secreted IL-8 was measured via ELISA. Results are representative of three separate experiments. *, δ.