Activation of Peroxisome Proliferator-Activated Receptor-Gamma by Glitazones Reduces the Expression and Release of Monocyte Chemoattractant Protein-1 in Human Mesothelial Cells
Figure 2
(a) Effect of rosiglitazone on mesothelial MCP-1 release. MC were stimulated with rosiglitazone in the given concentrations for 48 hours. MCP-1 antigen was measured in the cell culture supernatants using ELISA technique. *indicates a value < 0.05; **indicates a value < 0.01 (). (b) Effect of rosiglitazone on mesothelial MCP-1 mRNA levels. MC were incubated with rosiglitazone 10 μM for 4 hours. Total RNA was extracted and analysed via RT-PCR. MCP-1 mRNA levels were adjusted to the housekeeper rRNA and are expressed as relative to the control. The figure is a representative of three independent experiments.
