Activation of Peroxisome Proliferator-Activated Receptor-Gamma by Glitazones Reduces the Expression and Release of Monocyte Chemoattractant Protein-1 in Human Mesothelial Cells
Figure 4
(a) PPAR--dependency of the rosiglitazone effect on mesothelial MCP-1 release. MC were preincubated with GW-9662 10 μM for 24 hours (or with control media) and then incubated with the given conditions for 8 hours. Cell supernatants were examined by ELISA. indicates a value < 0.05 (). (b) PPAR--dependency of the rosiglitazone effect on mesothelial MCP-1 mRNA expression. MC were preincubated with GW-9662 10 μM for 24 hours (or with control media) and then incubated with the given conditions for 8 hours. Total RNA was extracted and examined by RT-PCR. The figure shows a representative experiment out of three independent ones.
