Essential Role of Mast Cells in the Visceral Hyperalgesia Induced by T. spiralis Infection and Stress in Rats
Figure 2
The increased PAR2 expression induced by intestinal infection (PI) and acute cold restraint stress (ACRS) in distal colon of +/+, but not Ws/Ws, rats. (a) Representative western blotting for PAR2 in extracts from colon tissue. (b) Quantitative analysis of PAR2 protein. Data was expressed as normalized density to β-actin. (c) Relative levels of PAR2 mRNA in colon tissue. Data was normalized to 18S ribosomal RNA and expressed using the 2−ΔΔCt method. ; .