Essential Role of Mast Cells in the Visceral Hyperalgesia Induced by T. spiralis Infection and Stress in Rats
Figure 4
Role of ERK phosphorylation in +/+ and Ws/Ws rats induced by intestinal infection (PI) and acute cold restraint stress (ACRS). (a–f) Representative immunofluorescence images of pERK1/2 immunoreactivity- (IR-) positive neurons in L6S1 DRGs in +/+ rats (a–c) and Ws/Ws rats (d–f). Scale bar: 100 μm. (g) Quantification of pERK1/2 IR labelling intensity in L6S1 DRGs. (h) Representative western blot for phosphorylated ERK1/2 in L6S1 DRG extracts using a phospho-ERK specific Ab (pERK1/2, upper panel). Protein loading was confirmed by reprobing the membrane with ERK1/2 Ab (lower panel). (i) Quantitative analysis of phosphorylated ERK1/2 protein. Data was expressed as normalized density to ERK1/2. *; **.
