Research Article

Elevated Circulating Interleukin-27 in Patients with Coronary Artery Disease Is Associated with Dendritic Cells, Oxidized Low-Density Lipoprotein, and Severity of Coronary Artery Stenosis

Figure 3

ox-LDL induced interleukin (IL)-27 production in monocyte-derived DCs. (a) Immature DCs were stimulated with different concentrations of ox-LDL for 24 h. The concentrations of IL-27 were analyzed using ELISA kit. (b) and (c) The mRNA expression levels of IL-27 p28 and ebi3 were evaluated by a real-time PCR analysis. The results are expressed as relative level to the control value. Results shown are means ± SD of six experiments. (d) and (e) Immature DCs were stimulated with ox-LDL (100 mg/mL) for 0, 8 h, 16 h, and 24 h. Then the mRNA expression levels of IL-27 p28 and ebi3 were evaluated by a real-time PCR analysis. The results are expressed as relative level to the control value. Results shown are means ± SD of six experiments.
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