Immunophenotyping of Posttraumatic Neutrophils on a Routine Haematology Analyser
Figure 1
Morphological display of leukocytes. Representative example of the morphological plots of a blood sample from a healthy volunteer on (a) a FACSCalibur and (b) a Cell-Dyn Sapphire. The cells in red are the neutrophils, the purple population are the monocytes, the blue cells are lymphocytes, and the puple in green is debris. (a) Forward Scatter (FSC)—Side Scatter (SSC) display from a FACSCalibur. FSC represents the size/cell volume of cells, SSC represents the inner complexity of the particle. (b) Multi Angle Polarised Scatter Separation (MAPSS) leukocyte differential plot from a Cell-Dyn Sapphire. Axial Light Loss (ALL) is an indicator of cell size, Intermediate Angle Scatter (IAS) represents the cell complexity.
