Immunophenotyping of Posttraumatic Neutrophils on a Routine Haematology Analyser
Figure 3
Incubation of monoclonal antibodies by the Cell-Dyn Sapphire. In ten healthy volunteers, two blood samples were taken. Half of the whole blood samples () were preincubated for 5 minutes with fMLF 10−6, and half of samples () were not preincubated. The samples were then put in a Cell-Dyn Sapphire, and this analyser fully automatic performed flow cytometry analysis with the monoclonal phage antibody A27 that recognizes the active FcγRII (represented as expression of active FcγRII). MFI = median fluorescence intensity.
