Anti-Inflammatory Properties of Sirtuin 6 in Human Umbilical Vein Endothelial Cells
Figure 4
Effect of SIRT6 knockdown on COX-prostaglandin pathway. HUVECs were transfected with 50 nM SIRT6 or NS siRNA for 72 h followed by 10 g/mL LPS for 24 h (). (a) COX-1 and COX-2 mRNA expression was quantified by qRT-PCR. (b) PGE2 and levels in the conditioned media was assayed using EIA. Each bar represents the mean SEM. versus NS siRNA + LPS.
