Anti-Inflammatory Properties of Sirtuin 6 in Human Umbilical Vein Endothelial Cells
Figure 6
Effect of SIRT6 knockdown on cell adhesion. HUVECs were transfected with 50 nM SIRT6 or NS siRNA for 72 h followed by 10 g/mL LPS for 24 h (). (a) The gene expression of the adhesion molecules VCAM-1, ICAM-1, and P-selectin was quantified by qRT-PCR. (b) ICAM-1 concentration in the conditioned media was assayed using ELISA. (c) Transfected HUVECs were plated onto gelatin-coated 96-well plates for 1.5 h, and cell adhesion assays were performed using MTT. Extracted dye was quantified by absorbance at 570 nm. Each bar represents the mean SEM. versus NS siRNA + LPS. (d) PMN cells were added to each well and were incubated at 37°C for 60 min. The number of adhered PMNs was evaluated by a colorimetric assay using MTT assay and quantification by absorbance at 570 nm. Each bar represents the mean SEM. versus NS siRNA + LPS.
