Anti-Inflammatory Properties of Sirtuin 6 in Human Umbilical Vein Endothelial Cells
Figure 7
Effect of SIRT6 siRNA on HUVEC angiogenic proteins and cell migration. HUVECs were transfected with 50 nM SIRT6 or NS siRNA for 72 h followed by 10 g/mL LPS for 24 h (). (a) The gene expression of the proangiogenic proteins VEGF and FGF-2 was quantified by qRT-PCR. Each bar represents the mean SEM. versus NS siRNA + LPS. (b) Transfected HUVECs were put into transwell inserts coated on the underside with 0.1% gelatin. The total number of migrating cells were counted after 5 h at 37°C. Fold change was calculated relative to NS siRNA transfected cells. Each bar represents the mean SEM. versus NS siRNA + LPS.
