Anti-Inflammatory Properties of Sirtuin 6 in Human Umbilical Vein Endothelial Cells
Figure 8
SIRT6 regulates inflammation via NF-B. (a) HUVECs were transfected with 50 nM SIRT6 or NS siRNA for 96 h (). NF-B p65 mRNA expression was quantified by qRT-PCR. Each bar represents the mean SEM. versus NS siRNA transfected cells. (b) HUVECs were cotransfected with 1 g negative control construct and NF-B reporter construct, along with 1 g empty or SIRT6 pCMV6 vector for 72 h. Promoter activity is expressed as a ratio of luciferase activity of the control group, which was set as 1. Each bar represents the mean SEM ( experiments). versus cells transfected with NF-B reporter plus empty pCMV6 vector.
