Changes in the Monocytic Subsets CD14^dimCD16⁺ and CD14⁺⁺CD16⁻ in Chronic Systolic Heart Failure Patients

Figure 1

Characterization of monocyte subsets in HF patients and controls. (a) Representative flow cytometry dot plots of monocyte subsets in a healthy control and an HF patient, including the median values of each subset. Monocytes were gated by their side and forward scattering, and (b) their percentage from total blood leukocytes was determined; monocytes that were positive for HLA-DR expression were separated into three subsets according to their expression of CD14 and CD16, and their percentage of the total monocytes was determined in (c) CD14⁺CD16⁺ monocytes (gated R2 in (a), the green subpopulation), (d) CD14⁺⁺CD16⁻ monocytes (gated R1 in (a), the red subpopulation), and (e) CD14^dimCD16⁺ monocytes (gated R3 in (a), the blue subpopulation).

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