CRP and TNF-𝜶Induce PAPP-A Expression in Human Peripheral Blood Mononuclear Cells

Figure 1

PBMCs were stimulated with CRP or TNF-α for 2, 8, 16, 24 hours. (a) PAPP-A mRNA were measured using RT-PCR. (b) PAPP-A protein expression were measured by western blotting. (c) Culture supernatant was collected respectively to analyze PAPP-A concentration by an ultra-sensitive ELISA. The resultant data are expressed as the means ± SD of six donors. *: 𝑃<0.05 compared with the control. #: 𝑃<0.05 compared with the PBMCs stimulated with CRP for 24 hours; ▲: 𝑃<0.05 compared with the PBMCs stimulated withTNF-α for 2 hours or 8 hours.

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