Anti-Inflammatory Effects of Adrenomedullin on Acute Lung Injury Induced by Carrageenan in Mice

Figure 2

Effect of adrenomedullin (AM) on the immunohistochemical localization of ICAM-1 and P-selectin. No positive staining for ICAM-1 was observed in lung sections taken from sham mice treated with AM (a). Lung sections taken from CAR-treated mice showed intense positive staining for ICAM-1 along the vessels (b). The degree of positive staining for ICAM-1 was markedly reduced in lung sections obtained from mice treated with AM (c). No positive staining for P-selectin was observed in lung sections taken from sham mice (d). Lung sections taken from CAR-treated mice treated with vehicle showed intense positive staining for P-selectin along the vessels (e). The degree of positive staining for P-selectin was markedly reduced in tissue sections obtained from mice treated with AM (f). The figure is representative of at least three experiments performed on different experimental days. A significant increase in ICAM (g, g1) and P-selectin (h, h1) quantification, assayed by western blot analysis, was detected in lungs obtained from mice subjected to CAR-induced pleurisy, if compared with lung from sham mice (g, g1 and h, h1). Treatment with AM significantly attenuated these proteins quantification in the lung tissues (g, g1 and h, h1). A representative blot of lysates obtained from 5 animals per group is shown and densitometry analysis of all animals is reported. The results in panel (g1) and (h1) are expressed as mean ± s.e.m. from 𝑛=5/6 lung tissues for each group. ^∗𝑃<0.01 versus sham group. ^∘𝑃<0.01 versus CAR.