Anti-Inflammatory Effects of Adrenomedullin on Acute Lung Injury Induced by Carrageenan in Mice

Figure 4

Effect of adrenomedullin (AM) on nitrotyrosine formation, lipid peroxidation, and PARP activation. No staining for nitrotyrosine is present in lung section from sham mice (a). Lung sections taken from CAR-treated mice treated with vehicle showed positive staining for nitrotyrosine, localized mainly in inflammatory cells (b). There was a marked reduction in the immunostaining for nitrotyrosine in the lungs of CAR-treated mice treated with AM (c). Malondialdehyde (MDA) levels, an index of lipid peroxidation, were significantly increased in lung tissues 4 h after CAR administration (h), if compared with lung from sham mice (h). AM significantly reduced the CAR-induced elevation of MDA tissue levels (h). Lung sections taken from CAR-treated mice showed positive staining for PAR (f). There was a marked reduction in the immunostaining for PAR in the lungs of CAR-treated mice treated with AM (g). Lung section from sham mice showed no staining for PAR (e). Densitometry analysis of immunocytochemistry photographs (𝑛=5 photos from each sample collected from all mice in each experimental group) for nitrotyrosine and PAR from lung tissues was assessed (d). The assay was carried out by using Optilab Graftek software on a Macintosh personal computer (CPU G3-266). Data are expressed as % of total tissue area. The figure is representative of at least 3 experiments performed on different experimental days. Data are expressed as mean ± s.e.m. from 𝑛=10 mice for each group. ^∗𝑃<0.01 versus sham group. ^∘𝑃<0.01 versus CAR.