The Impact of Various Reactive Oxygen Species on the Formation of Neutrophil Extracellular Traps

Figure 1

Generation and inhibition of ROS in neutrophils. The generation of superoxide (O₂^•−) from oxygen (O₂) is mediated either by the NADPH oxidase complex (NOX) or in mitochondria by cytochrome c peroxidase or xanthine oxidase. Superoxide will be converted to hydrogen peroxide (H₂O₂) either spontaneously or mediated by superoxide dismutase (SOD). Hydrogen peroxide is a source of hydroxyl radical (OH^•) via the Fenton reaction. Myeloperoxidase uses hydrogen peroxide as substrate for the formation of halogenated ROS such as hypochlorite (OCl⁻). Reaction of hypochlorite with hydrogen peroxide will result in the formation of singlet oxygen (¹O₂). The effect of the inhibitors used in the study (10 μM Rotenone, 10 μM Dinitrophenol, 500 nM FCCP, 20 μM DPI, 38 μM Aroclor, 10 μM DETC, 200 μM Dipyrone and 200 μM Aminopyrine) is indicated.