The Impact of Various Reactive Oxygen Species on the Formation of Neutrophil Extracellular Traps
Figure 4
Extracellular ROS production of neutrophils incubated with various inhibitors as measured by the lucigenin-amplified chemiluminescence assay. Freshly isolated human neutrophils were incubated with or without inhibitors (10 μM Rotenone, 10 μM Dinitrophenol, 500 nM FCCP, 20 μM DPI, 38 μM Aroclor, 10 μM DETC, 200 μM Dipyrone, and 200 μM Aminopyrine) for 30 min at 37°C. Subsequently, lucigenin was added, and the ROS production was induced with 20 nM PMA. The -release was detected by measuring chemiluminescence for a period of 1 h at 37°C. The control without PMA shows the -release of unstimulated neutrophils. (a) Time kinetics of -release (chemiluminescence) in one representative experiment. (b) -release quantified by calculation of the area under the curve (AUC). Data show mean ± SD from four independent experiments, **: ; *: as compared to the PMA-stimulated sample without inhibitor (Medium).
