Adipose Tissue-Specific Deletion of 12/15-Lipoxygenase Protects Mice from the Consequences of a High-Fat Diet

Figure 5

Fat-specific 12/15-LO-deficient mice exhibit improvements in pancreatic islet health. (a) Immunohistochemistry for insulin expression on pancreatic sections from mice after the 16-week feeding regimen was analyzed for average islet area, average islet area per pancreas area, and average insulin staining per islet; 𝑛 = 3–5; Chow: chow diet; HFD: high-fat diet; wt: wild type 12/15-𝐿𝑂^{𝑙𝑜𝑥𝑃/𝑙𝑜𝑥𝑃}. (b) mRNA measurements of key proinflammatory genes were examined by RT-PCR in isolated pancreatic islets from mice after the 16-week feeding regimen; 𝑛 = 6–8; HFD: high-fat diet; wt: wild type C57BL/6J. All mRNA data were normalized to total actin, and the fold changes in expression were calculated relative to control. All data represent the means ± SEM. ^∗𝑃<0.05 and ^†𝑃<0.02 versus control, unless otherwise indicated.

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