Anesthetic Isoflurane Posttreatment Attenuates Experimental Lung Injury by Inhibiting Inflammation and Apoptosis

Figure 4

Treatment with 1.4% ISO downregulates proinflammatory cytokine levels in lung tissues and BALF from ZY-challenged mice. (a) RT-PCR analysis for the expression of TNF-α, IL-1β, IL-6, and MIP-2 mRNA; (b)–(e) amplicons were densitometrically quantified, and bars represent the relative amounts of amplified TNF-α, IL-1β, IL-6, and MIP-2 mRNA normalized against β-actin; (f) BALF TNF-α; (g) BALF IL-1β; (h) BALF IL-6; (i) BALF MIP-2. BALF was obtained to measure these indicators using ELISA kits. The respective densitometric analysis of mRNA bands is from three separate experiments. The animals were treated as described in Figure 1. Data are mean ± SD (𝑛=10 mice per group). *𝑃<0.05 versus sham; ^#𝑃<0.05 versus ZY + vehicle.

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