Activation of Protease-Activated Receptor 2-Mediated Signaling by Mast Cell Tryptase Modulates Cytokine Production in Primary Cultured Astrocytes
Figure 4
Effects of inhibitors of cell signaling pathways on tryptase (Tt)-induced secretion of IL-6 (a) and TNF-α (b) from astrocytes. Astrocytes were exposed to 1 μg/mL of tryptase in the presence or absence of SB203580 (SB, 20 μM), PD98059 (PD, 20 μM), SP600125 (SP, 20 μM), and LY294002 (LY, 20 μM) at 37°C for 24 h before culture supernatant being collected. Data are presented as the mean ± SEM of four independent experiments. versus control groups (Con), versus corresponding tryptase treatment groups.
