PGE2 Desensitizes β-Agonist Effect on Human Lung Fibroblast-Mediated Collagen Gel Contraction through Upregulating PDE4
Figure 1
Synergistic effect of salmeterol and cilomilast on lung fibroblast-mediated collagen gel contraction. HFL-1 cells were cast into collagen gels and released into SF-DMEM containing either varying concentrations of salmeterol (0, 10−8, 10−7 and 10−6 M, (a)) or cilomilast (0, 10−7, 10−6, and 10−5 M, (b)) as indicated. Gel size was measured on day 2. Vertical axes: gel size was expressed as percentage of initial size on day 2 (%); horizontal axes: concentrations of salmeterol or cilomilast. *. (c) Synergistic effect of salmeterol and cilomilast are shown. HFL-1 cells were cast into collagen gels and released into SF-DMEM containing salmeterol (10−8 M) and/or cilomilast (10−6 M). Gel size was measured daily for 5 days. Vertical axis: gel size was expressed as percentage of initial size (%); horizontal axis: time (day). ** compared to SF-DMEM at each time point.
