PGE2 Desensitizes β-Agonist Effect on Human Lung Fibroblast-Mediated Collagen Gel Contraction through Upregulating PDE4
Figure 2
Cilomilast potentiates the inhibitory effect of salmeterol on fibroblast-mediated collagen gel contraction. (a) Effect of cilomilast on varying concentrations of salmeterol. HFL-1 cells were cast into collagen gels and released into SF-DMEM containing varying concentrations (0, 10−8, 10−7, and 10−6 M) of salmeterol in the presence or absence of cilomilast (10−6 M). Vertical axis: gel size on day 2 (%); horizontal axis: salmeterol concentration (M). Square: SF-DMEM only; triangle: in the presence of 10−6 M cilomilast. *; ** compared to salmeterol only. (b) Effect of salmeterol in the presence of varying concentrations of cilomilast. HFL-1 cells containing collagen gels were released into SF-DMEM supplemented with 10−8 M salmeterol in the presence or absence of varying concentrations (0, 10−7, 10−6, and 10−5 M) of cilomilast. Vertical axis: gel size on day 2 (%); horizontal axis: cilomilast concentrations (M). Square: SF-DMEM only; triangle: in the presence of salmeterol (10−6 M). ** compared to cilomilast alone at each time point.
