PGE2 Desensitizes β-Agonist Effect on Human Lung Fibroblast-Mediated Collagen Gel Contraction through Upregulating PDE4
Figure 4
Exogenous PGE2 pretreatment potentiates cilomilast but not salmeterol. Confluent HFL-1 cells were pretreated with SF-DMEM with or without 10−9 M PGE2 for 24 hrs. The next day, cells were cast into collagen gels as described in Section 2. (a) PGE2 pretreatment abolished the inhibitory effect of salmeterol on collagen gel contraction. (b) PGE2 pretreatment potentiated the effect of cilomilast in inhibiting collagen gel contraction. (c) Effect of PGE2 pretreatment on the synergistic effect of salmeterol and cilomilast.
