PGE2 Desensitizes β-Agonist Effect on Human Lung Fibroblast-Mediated Collagen Gel Contraction through Upregulating PDE4
Figure 5
Effect of PGE2 and indomethacin on PDE4 expression. Confluent HFL-1 cells were treated with SF-DMEM with or without 10−9 M PGE2 or 10−6 M indomethacin for 24 hrs. Whole cell lysates were immunoblotted for PDE4 isoforms and -actin as loading control. Images were visualized with ECL plus (a), and the bands from 3 separate experiments were quantified (b). *.
