TNF-𝜶 Regulates Natriuretic Peptides and Aquaporins in Human Bronchial Epithelial Cells BEAS-2B

Figure 3

TNF-α modulation of NPs and NPR-1 may require activation of NF-𝜅B pathway. BEAS-2B cells were treated with 10, 20, and 40 ng/mL TNF-α alone or in combination with NF-𝜅B inhibitor BAY 11-7082 (1 μM), for 24 h. ((a), (b)) ANP, ((c), (d)) BNP, ((e), (f)) NPR-1 gene expression at mRNA ((a), (c), and (e)) and protein level ((b), (d), (f)), and (g) Phospho-I𝜅B-α protein level. Western blots were obtained by using the specific rabbit polyclonal or monoclonal Abs. The blots were stripped of the bound Ab and reprobed with mouse anti-β-actin, to confirm equal loading. Western blots are representative of three separate experiments. All histograms indicate means±SD of three different cultures each of one tested in quadruplicate and expressed as percentage of control (^∗𝑃<0.05, ^∗∗𝑃<0.01, ^∗∗∗∗𝑃<0.0001 versus Controls). (^∘𝑃<0.05, ^∘∘𝑃<0.01, and ^∘∘∘∘𝑃<0.0001 versus BAY). C: Controls (untreated cells).

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