TNF-𝜶 Regulates Natriuretic Peptides and Aquaporins in Human Bronchial Epithelial Cells BEAS-2B

Figure 4

TNF-α modulation of protein levels of BNP and NPR-1 may require activation of NF-𝜅B pathway. BEAS-2B cells were treated 10 ng/mL TNF-α alone or in combination with the inhibitor QNZ (10 μM), for 24 h. ((a), (b)) BNP, ((c), (d)) NPR-1 gene expression at mRNA ((a), (c)) and protein ((b), (d)) level, and (e) Phospho-I𝜅B-α protein level. Western blots were obtained by using the specific rabbit polyclonal or monoclonal Abs. The blots were stripped of the bound Ab and reprobed with mouse anti-β-actin, to confirm equal loading. Western blots are representative of three separate experiments. All histograms indicate means±SD of three different cultures each one tested in quadruplicate and expressed as percentage of control (^∗∗𝑃<0.01, and ^∗∗∗∗𝑃<0.0001 versus Controls). (^∘∘𝑃<0.01 versus QNZ). C: Controls (untreated cells).

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