TNF-𝜶 Regulates Natriuretic Peptides and Aquaporins in Human Bronchial Epithelial Cells BEAS-2B

Figure 6

TNF-α decreases AQP1 and AQP5 expression in BEAS 2B cells. BEAS-2B cells were treated with 10, 20, and 40 ng/mL TNF-α for 24 h. Gene expression of AQP1 ((a), (b)) and AQP5 ((c), (d)) at mRNA ((a), (c)) and protein ((b), (d)) level. Western blots were obtained by using the specific rabbit polyclonal Abs. The blots were stripped of the bound Ab and reprobed with mouse anti-β-actin, to confirm equal loading. Western blots are representative of three separate experiments. All histograms indicate means±SD of three different cultures each one tested in quadruplicate and expressed as percentage of control (^∗𝑃<0.01, ^∗∗𝑃<0.001, and ^∗∗∗𝑃<0.0001 versus untreated cells).

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