TNF-𝜶 Regulates Natriuretic Peptides and Aquaporins in Human Bronchial Epithelial Cells BEAS-2B

Table 1

Primer sequences, primer concentrations, and qRT-PCR cycling conditions.

Gene	Primer sequences	Primer concentrations	qRT-PCR cycling conditions
ANP	F: 5′-tcagcccagcccagagag-3′	200 nM	1 cycle at 95°C for 10 min; 40 cycles at 95°C for 1 min,
	R: 5′-gctccaatcctgtccatcctg-3′	200 nM	60°C for 30 sec and 72°C for 30 sec.
BNP	F: 5′-gagggcaggtgggaagcaaac-3′	200 nM	1 cycle at 95°C for 10 min, 40 cycles at 95°C for 1 min,
	R: 5′-gcaagaagagcaggagcaggag-3′	200 nM	60°C for 30 sec and 72°C for 30 sec.
NPR-1	F: 5′-ccctggaggtgctggctttgg-3′	200 nM	1 cycle at 95°C for 10 min; 40 cycles at 95°C for 1 min,
	R: 5′-ctctcaaggctactgggctcaacg-3′	200 nM	59°C for 30 sec and 72°C for 30 sec.
AQP1	F: 5′-ttggacacctcctggctattgact-3′	400 nM	1 cycle at 95°C for 10 min; 40 cycles at 95°C for 1 min,
	R: 5′-ccagtggttgctgaagttgtgtgt-3′	400 nM	60°C for 30 sec and 72°C for 30 sec.
AQP5	F: 5′-cgctcaacaacaacacaacgc-3′	200 nM	1 cycle at 95°C for 10 min; 40 cycles at 95°C for 1 min,
	R: 5′-ccagtgaagtagattccgacaagg-3′	200 nM	59°C for 30 sec and 72°C for 30 sec.
ACTB	F: 5′-cactcttccagccttccttcc-3′	600 nM	1 cycle at 95°C for 10 min; 40 cycles at 95°C for 1 min,
	R: 5′-acagcactgtgttggcgtac-3′	600 nM	59°C or 60°C for 30 sec and 72°C for 30 sec.

qRT-PCR: quantitative real time polymerase chain reaction; F: forward; R: reverse.